This algorithm is trained on in vitro binding assays, and peptides with predicted affinity (IC50) ≤ 500 nM are expected to be presented by the corresponding HLA class I. Similar to the HIV-1 ELISpot data, the predicted dengue repertoire size showed a negative correlation with TD of HLA class I allotypes (Fig. 2C and Table 1), which reached significance for HLA-B (r = −0.65, P = 0.02), HLA-A (r = −0.46, P = 0.05), and HLA-A/B allotypes combined (r = −0.53, P = 0.003). Here, HLA-A is linked to dengue disease.