Treatment of HepG2 cells with 2 mM FFA induced the overexpression of steatosis associated C/EBPα (untreated cells (UC): 1.24 ± 0.82; 0 μg/mL: 9.91 ± 0.88), FAS (UC: 1.34 ± 0.94; 0 μg/mL: 4.1 ± 0.48), SREBP1c (UC: 0.61 ± 0.19; 0 μg/mL: 1.84 ± 0.53), and CPT1A (UC: 1.17 ± 0.64; 0 μg/mL: 5.43 ± 0.56; Figure 5A–D). This evidence concerns the gene SREBF1 and steatosis.