For the breast cancer lines MCF-7 (hormone receptor positive), MDA-MB-453 (HER2 positive), SUM-190 (inflammatory breast cancer, HER2-positive) and MDA-MB-231 (partial mesenchymal, triple-negative breast cancer), as the number of spiked cells into HDB increased, the number of gene transcripts detected also increased (S1 Fig in S1 File). Here, NR4A1 is linked to triple-negative breast carcinoma.