One heterozygous missense variant (p.Pro13Thr) in POU5F1 was reported in one Chinese POI woman [19], and herein, one novel heterozygous variant in POU5F1 (c.133C>T;p.Pro45Ser), combined with a second novel heterozygous variant in the HK3 gene (Table 3), was found in a 19-year-old woman presenting with secondary amenorrhea, cubitus valgus, and hearing loss (POI-3) (Table 1). Here, HK3 is linked to sensory perception of sound.