To further explore the biological function of IGF2BP3 in bladder cancer, three IGF2BP3‐specific shRNAs were used to construct IGF2BP3 knockdown cells in T24 and UMUC3 cell lines, which had relatively higher IGF2BP3 expression, and IGF2BP3 was ectopically overexpressed in 5637 and J82 cell lines, which displayed relatively lower IGF2BP3 expression. Here, IGF2BP3 is linked to urinary bladder carcinoma.