Gain- and loss-of-function assays were performed to explore the effects of the lncRNA MAFG-AS1/miR-149-3p/HOXB8 axis in the biological functions of CRC cells by co-silence of lncRNA MAFG-AS1 and miR-149-3p as well as by silence of lncRNA MAFG-AS1 together with overexpression of HOXB8. The gene discussed is HOXB8; the disease is colorectal carcinoma.