Further regulatory mechanism exploration in our study demonstrated that lncRNA MAFG-AS1 could target and negatively regulate miR-149-3p and miR-149-3p could further bind to HOXB8 while lncRNA MAFG-AS1 functioned in CRC by mediating the expression of HOXB8 via miR-149-3p. This evidence concerns the gene MAFG and colorectal carcinoma.