After adjusting confounding factors such as BMI, bE2, infertility duration, number of pregnancies, LH on the trigger day, number of oocytes retrieved, and endometrial thickness on trigger day, multivariate logistic regression analysis showed that age was an independent risk to significantly (P < 0.05) affect the clinical pregnancy rate (OR = 0.940, 95% CI: 0.919, 0.961), live birth rate (OR = 0.961,95% CI: 0.943, 0.979), and miscarriage rate (OR = 1.133, 95% CI:1.060, 1.212) (Table 2). Here, PLOD1 is linked to spontaneous abortion.