To clarify the biological functions of TIM in breast cancer, the TIM expression in ER-positive breast cancer cell lines MCF7, T47D, and ZR75-30, ER-negative breast cancer cell lines Bcap37, SH-BR-3, MDA-MB-231, and MDA-MB-468, and normal mammary epithelial cell line HBL100 was tested by both qPCR and western blotting (WB) (Fig. 2a, b). Here, TIMELESS is linked to breast carcinoma.