As shown in Fig. 4a, The size and weight of tumors in the sh-MNX1-AS1 group were significantly smaller than those in the nc-MNX1-AS1 group (4-week volume: 0.86 ± 0.16 vs. 1.92 ± 0.16 cm3; weight: 0.33 ± 0.08 vs. 0.81 ± 0.11 g, P < 0.05), which indicated that the proliferation ability of tumor in vivo was markedly decreased after knockdown of MNX1-AS1. This evidence concerns the gene MNX1 and neoplasm.