However, after transfection for 72 h, the expression of OCN, ALP, and COL 1 was reduced by 63.2% (P < 0.01), 52% (P < 0.01), and 53.2% (P < 0.01) in ligament fibroblasts from patients with OPLL compared with 3.1% (P > 0.01), 6.8% (P > 0.01), and 10.7% (P > 0.01) in the non-OPLL patients, respectively (Fig. 5b–d). Here, BGLAP is linked to ossification of the posterior longitudinal ligament of the spine.