RPS28 and cancer: We also observed downregulation of tRFs which although they exhibited fold change they were not among the statistically significant, but have been previously reported with regulatory roles like tRF-3011a (deriving again from tRNA-Leu-CAG), which is known to regulate the expression levels of the ribosomal protein RPS28 and tRF-1001 which was among the first tRFs to be associated with cancer (Figure 2A,B) [18,23].