Comparative analysis of altered metabolites and proteins by IPA indicated that elevation of numerous glucose metabolites was associated with increase of expression of enzymes involved in glycolysis, gluconeogenesis and lactate synthesis from pyruvate, such as glucose-6-phosphate isomerase, fructose-1,6-bisphosphatase, and lactate dehydrogenase A, in NASH-associated TSOD mice HCCs. The gene discussed is FBP1; the disease is metabolic dysfunction-associated steatohepatitis.