At 21 days post-infection, we found that the immunoreactivity for the neuronal marker microtubule associated protein 2 (MAP2) was progressively decreased in the HSV-1- infected NSCs in a dose-dependent manner (Fig 2E and 2F), thus suggesting that HSV-1 infection markedly altered the neurogenic differentiation, at least, impaired the neuronal differentiation. Here, MAP2 is linked to infection.