PI4KIIIβ protects lipid-overexposed cardiomyocytes from developing insulin resistance. For examining PI4KIIIβ’s cardioprotective potential in cardiomyocytes during lipid overexposure, we started out with culturing of aRCMs for 48 h with adenoviral PKD1 vector in the presence or absence of MI14, followed by 30 min recovery, short-term (15 min) insulin stimulation, and finally, measurement of glucose uptake. The gene discussed is PKD1; the disease is Insulin resistance.