We would like to emphasize that this did not affect mutations in driver genes such as BRAF or KRAS. This is in line with data from single cell subcloning of established CRC cell lines SW480 and HT29, which showed consistent mutations of KRAS and BRAF, respectively, across all established single cell clones—despite ongoing karyotype evolution/chromosomal instability16. This evidence concerns the gene BRAF and colorectal carcinoma.