To explore whether WNT3 induction is responsible for the activation of the WNT/β-catenin signaling pathway and subsequent CSC activation in 5-FU-treated CRC, we treated tumor organoids derived from ApcMin/+/Lgr5EGFP mice with porcupine inhibitor LGK-97432 and 5-FU. The gene discussed is WNT3; the disease is colorectal carcinoma.