WNT3 and colorectal carcinoma: Effective suppression of 5-FU-induced WNT3 secretion (Fig. 5b) and inhibition of 5-FU-induced activation of the WNT/β-catenin signaling pathway and CSC were observed following LGK-974 co-treatment with 5-FU in the HCT116 human CRC cell line, as demonstrated by protein and mRNA levels of β-catenin and CSC markers, respectively (Fig. 5c, d).