LGR5 and colorectal carcinoma: Moreover, 5-FU treatment of mouse tumor organoids derived from ApcMin/+/K-RasG12DLA2/Lgr5EGFP mice and in vivo injection of 5-FU to ApcMin/+/K-RasG12DLA2/Lgr5EGFP mice confirmed increased expression of β-catenin and Lgr5 (GFP), showing that 5-FU-induced activation of the Wnt/β-catenin signaling pathway and CSCs also occur in an advanced murine CRC model (Supplementary Fig. 2a, b).