Mel888 melanoma cells (VGLL1-negative, HLA-A*0101-positive) pulsed with titrated amounts of VGLL1 peptide elicited recognition and killing at peptide concentrations as low as 10 nM, indicating relatively high affinity for cognate peptide (Fig. 4d and Supplementary Fig. 16). This evidence concerns the gene VGLL1 and melanoma.