FBN1 and Marfan syndrome: Twenty-two MFS relative genes were screened by next-generation sequencing for variant seeking (NovaSeq 6000, Illumina, USA), and large fragment deletions or repeated variants of the FBN1 gene were detected by multiplex ligation-dependent probe amplification (MLPA) following the protocol of the SALSA MLPA P065 Marfan Syndrome-1 probemix and P066 Marfan Syndrome-2 probemix kit (MRC-Holland).