CXCL8 and cancer: To test this, we employed MDA-MB-231 cells, a cancer cell line that naturally overexpresses HRGβ2 and IL-8, and a clonal derivative of this cell line stably transfected with an antisense HRGβ2 construct (MDA-MB-231/AS-31) [30], leading to almost undetectable HRGβ2 expression [30,31,32,33].