NFKB1 and central nervous system cancer: Figure 3D shows that psiCHECK-2-proDGCR5 luciferase activity was significantly decreased via the overexpression of NF-κB1. Mutating the predicted NF-κB1 RE in the DGCR5 promoter region abolished the alterations in luciferase activity (Figure 3D). Therefore, considering that NF-κB1 exerts an essential effect on the mediation of various major characteristics related to mesenchymal differentiation [29], we hypothesize that DGCR5 exerts an effect on mesenchymal differentiation] in glioma.