In addition, five up‐regulated genes, including ZBTB20, RASA1, POU2F1, NFAT5 and PDE1C were targeted by three miRNAs and 23 genes including APBB2, CAMSAP2 and DICER1 were targeted by two miRNAs, but their functions in GDM were poorly understood. Here, ZBTB20 is linked to gestational diabetes.