Notably, 10 genes had LoF variants in 3 or more patients with CHD and NDD or CHD and ECA (supporting cohort size for statistics: CHD: 1482 individuals with ECA and 1393 with NDD; control: 9808 individuals; P < .05), including 2 CR regulatory genes (catenin beta 1 [CTNNB1; in 3 participants with CHD and ECA, 2 with CHD and NDD, and 0 control participants) and transcription factor 12 [TCF12; in 3 patients with CHD and NDD, 2 patients with CHD and ECA, and 2 control participants]; eTable 10 in the Supplement). This evidence concerns the gene CTNNB1 and Neurodevelopmental delay.