To explore potential downstream function(s) of RUNX1T1, we used lentivirus to stably overexpress RUNX1T1 in five lung cancer cell lines with low endogenous RUNX1T1 expression (A549, H16450, H841, SW1271, H1299) to create models of RUNX1T1 amplification. This evidence concerns the gene RUNX1T1 and lung cancer.