INSM1 and small cell lung carcinoma: Overexpression or knockout of RUNX1T1 in our cell line models did not produce obvious histologic changes or consistent alterations in the expression of several neuroendocrine (NE) markers (ASCL1, NeuroD1, synaptophysin, INSM1, and TTF1) assayed by western blot analysis, as might be expected since NE gene expression is a feature distinguishing SCLC from NSCLC (data not shown).