The study also established TFs-hub genes and miRNAs-hub genes networks, and found several reporter regulatory molecules (e.g., E2F1, PTTG1, TP53, ZBTB16, hsa-miR-130a-3p, hsa-miR-204-5p) significantly related to the progression and prognosis of breast cancer. This evidence concerns the gene ZBTB16 and breast cancer.