We also found that several reporter regulatory molecules (e.g., E2F1, TP53, PTTG1, ZBTB16, hsa-miR-340-5p, hsa-miR-130a-3p, hsa-miR-200b-3p, hsa-miR-204-5p) regulated the transcription or post-transcription of hub genes associated with major biological processes and pathways in breast cancer, and they were also related to the OS of breast cancer patients. This evidence concerns the gene ZBTB16 and breast cancer.