LCN2 and TWIST1-related craniosynostosis: The optimal cutoff point of NGAL to diagnose CRS1 was >353.23 ng/ml, the area under the AUC curve 0.732 (95% CI 0.65–0.80, p < 0.001), sensitivity 74.47%, specificity 68.48%, positive predictive value 54.7%, and negative predictive value 84%.