Plasma NGAL was valuable for the diagnosis of CRS1 with a cutoff point >353.23 ng/ml, the AUC curve was 0.732 (95% CI 0.65–0.80, p < 0.001), sensitivity 74.47%, specificity 68.48%, positive predictive value 54.7%, and negative predictive value 84%. This evidence concerns the gene LCN2 and TWIST1-related craniosynostosis.