Similarly, ROCKi pre-treated B16F10 melanoma cells (Supplementary Fig. 5b, c) injected subcutaneously into immunocompetent C57BL/6J mice showed reduced tumour growth (Supplementary Fig. 5d) and decreased amoeboid cell number in ROCKi pre-treated group (Supplementary Fig. 5e–g). The gene discussed is ROCK1; the disease is neoplasm.