In order to address the possibility that a ≥ 30% cutoff in primary IDHm astrocytomas might be too stringent, we performed additional K-M survival analysis on grade 2/3 tumors that would hypothetically be upgraded to grade 4 under the proposed cIMPACT-NOW 5/6 criteria using CDKN2A homozygous deletion thresholds of 10% and 20% tumor cells deleted (Fig. 5c and d). The gene discussed is CDKN2A; the disease is astrocytoma (excluding glioblastoma).