To demonstrate the tumor-targeting capabilities of DARP-Lip(Bn) and EC1-LoPE in living animals, HER2/EpCAM–overexpressing tumor-bearing athymic BALB/c Nude mice were administered i.p. with proteoliposomes (10 nM) or fusion toxin (40 μg) conjugated with Cy5.5 as described in Materials and Methods, and then assessed by a living-animal imaging technique (using IVIS Spectrum CT, Perkin Elmer, Waltham, MA, USA) at different time points. Here, ANKRD23 is linked to neoplasm.