To confirm that this NSP1-mediated repression occurs in human cells upon activation of double-stranded RNA (dsRNA)-sensing pathways typically triggered by viral infection, we treated a human lung epithelial cell line (A549) with poly(I:C), a molecule that is structurally similar to dsRNA and known to induce an antiviral innate immune response (Alexopoulou et al., 2001; Kato et al., 2006) (Figure S4K). Here, SH2D3A is linked to viral infectious disease.