Methylation analysis showed that the DNA methylation level of GADD45γ in SKM‐1 cell line was significantly higher than that in SKM‐1 control group with azacitidine added, while the expression level of GADD45γ in SKM‐1 cell line was significantly lower than that in SKM‐1 control group with azacitidine added, which was also confirmed in the GADD45γ overexpression system of SKM‐1 cell line, indicating that azacitidine can promote the expression of GADD45γ in MDS cell line, and the demethylation of GADD45γ could improve the expression level of GADD45γ. Here, GADD45G is linked to myelodysplastic syndrome.