Observation of the co‐cultured cells for 7 days demonstrated that the chemoresistant cells (A549‐ZNF300‐GFP, A549/DDP‐mCherry and A549/DDP‐shZNF300‐NC‐GFP) were more sensitive to cisplatin combined with ATRA or ICA compared to the chemosensitive cells (A549‐mCherry, A549‐ZNF300‐NC‐GFP and A549/DDP‐shZNF300‐GFP), suggesting that ATRA and ICA could enhance the anti‐tumour effect of cisplatin on the chemoresistant cells by inducing the differentiation of tumour cells. This evidence concerns the gene ZNF300 and neoplasm.