Moreover, the Western blotting and IHC staining of the key genes on the tumour tissues from subcutaneous xenograft further proved that p‐p38 MAPK, p‐ERK1/2 (encoded by genes MAPK14 and MAPK1/MAPK3, respectively) and CD61 were inhibited, while p15 and Nanog were upregulated in the tumour tissues of xenograft models injected with A549‐ZNF300 and A549/DDP‐shZNF300‐NC cells compared to that injected with A549‐ZNF300‐NC and A549/DDP‐shZNF300 cells (Figure 3E,F). This evidence concerns the gene MAPK3 and neoplasm.