Moreover, as compared with negative scramble control, overexpression of miR-342-3p led to reduced cellular proliferation as measured by MTS assay (P < 0.0001, Fig. 4b), increased cell death by trypan blue exclusion assay (P = 0.0063, Fig. 4c), but no significant difference by annexin V-positive cells (figure not shown), and hence, these tumor-suppressive properties were mediated via mechanisms other than apoptosis. This evidence concerns the gene ANXA5 and neoplasm.