GPC3 and intrahepatic cholangiocarcinoma: Regarding the statistical analysis of GPC3 immunopositive cells detected by ICC, our results documented that the used sole treatments significantly reduced GPC3 immunostaining in HepG2 cells after 48 h incubation in a concentration-dependent manner when compared with control untreated HepG2 cells, (p < 0.05, Figure 3B,C).