In the present study, we used RNA scope (R-Scope) in situ hybridization (ISH) and confirmed that PVT1 overexpression was significantly associated with larger tumor size, deeper tumor invasion, lymph node metastases, and a short survival duration in paired GAC and adjacent normal tissue samples from this large cohort of GAC patients (n = 792). This evidence concerns the gene PVT1 and neoplasm.