A separate study that used MSP to quantify APC, RUNX3, GSTP1 reported that APC was the only independent prognostic gene in the prediction of PCa death across two cohorts of RP patients, one before PSA-testing was widespread (1980s cohort: n = 216) and one after the introduction of PSA-testing (1990s cohort: n = 243); adjusting for source of tumour tissue, GS and follow-up duration [82]. This evidence concerns the gene KLK3 and posterior cortical atrophy.