We also investigated the ongoing biological role of PI3K/Akt/mTOR signalling pathway in the phosphorylation of vascular endothelial growth factor receptor 2 (VEGFR-2), or vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) expression, and angiogenesis induced in vitro by HPV16 E6 oncoprotein in human umbilical vein endothelial cells (HUVECs) and cervical cancer cells. The gene discussed is VEGFA; the disease is cervical carcinoma.