We then investigated whether miR‐133a regulated the LASP1/TGF‐β/Smad3 signaling pathway in vivo, and western blot results revealed that miR‐133a restoration suppressed the expression of LASP1, TGF‐β and p‐Smad3 (Fig 5e), suggesting that miR‐133a might inhibit the tumor growth through TGF‐β/Smad3 signaling pathway by targeting LASP1. This evidence concerns the gene SMAD3 and neoplasm.