G6PD and hyperinsulinemic hypoglycemia, familial, 4: Conclusion: To increase the detection rate of newborns at-risk of G6DP deficiency, we suggest adjusting the cut-off value of 6.0 to 7.0 U/gHb and applying the multiplex SNaPshot assay as the second tier test for all newborns whose G6PD activities are between borderline range (5.1–7.0 U/gHb) in newborn screening to reduce clinic referrals.