After asthma induction, the proportion of Treg cells and Treg-associated Foxp3 mRNA expression significantly decreased, whereas the proportions of Th2 cells (and Th2-associated GATA3 mRNA expression) as well as Th17 cells (and Th17-associated ROR-γt mRNA expression) significantly increased in PBS/OVA mice relative to the control group (p < 0.05, Supplementary Figures 3A–B). This evidence concerns the gene GATA3 and asthma.