However, simple exposure to 1 μg LPS without asthma induction (LPS/PBS) did not significantly affect Foxp3, GATA3, or ROR-γt mRNA levels nor IL-10, TGF-β, IL-4, IL-5, IL-13, or IL-17 levels (p > 0.05, Supplementary Figures 3A–C) compared to those of Control mice. This evidence concerns the gene IL4 and asthma.