The fusion of autophagy and lysosome was further confirmed in Ziyu II-treated CRC cells, and used a tandem monomeric mRFP-GFP tagged LC3 construct, and noticed that the formation of red fluorescence autolysosomes(GFP-RFP+ signal) increased (Figures 4D–F). This evidence concerns the gene MAP1LC3A and colorectal carcinoma.