Interestingly, impairment of self-renewal similar to that in Klf4-deficient LSCs was reported in Tcf1–/–Lef1–/– CML, and one of the most strongly downregulated genes in this study was Klf4, suggesting that the observed effect in Tcf1–/–Lef1–/– CML could be mediated by loss of KLF461. This evidence concerns the gene LEF1 and chronic myelogenous leukemia, BCR-ABL1 positive.