The relative abundance of sgRNAs was measured using Illumina sequencing from the input library and the parasite DNA isolated from the early passage in HFFs (prior to BMDM infection), three additional passages on naïve (referred to as Naïve BMDM) or IFNγ-activated BMDMs (referred to as IFNγ BMDM), and the late passage in HFFs (referred to as HFF control). Here, IFNG is linked to infection.