In support of miR-22-3p downregulation with hepatic steatosis and/or inflammation, exposure of mouse primary hepatocytes (MPH), SK-Hep1 cells or human primary hepatocytes (HPH) to free fatty acids (e.g., oleate, palmitate or linoleate) ± inflammatory mediators (TNFα) also significantly reduced miR-22-3p expression (Figure S4). This evidence concerns the gene TNF and fatty liver disease.