To address this question, we used Karpas-299 cells, engineered to stably express the red fluorescent protein-enhanced green fluorescent protein-microtubule associated protein light chain 3 (RFP-EGFP-LC3) fusion protein, and measured the autophagic flux as an increase in the RFP/EGFP ratio—a method described by Gump et al. [54] that we recently validated in NPM-ALK+ ALCL [17]. The gene discussed is ALK; the disease is anaplastic large cell lymphoma.