Compared to mono-cultured BC cells, co-culture of BC cells and BCAFs increased the regulation of FSP1, TGF-β, PDGFβ, fibroblast growth factor 7 (FGF7), interleukins (IL-6 and IL-8), matrix metallopeptidases (MMP2 and MMP11), TIMP metallopeptidase inhibitor 1 (TIMP1), and vascular endothelial growth factor A (VEGFA). The gene discussed is TIMP1; the disease is breast cancer.