In our model, 80 ng/mL VEGF-A induced a moderate cellular effect with a diversified action based on conditions of HG or NG; considering that very high increase in VEGF-A levels have been reported in vitreous of patients with proliferative diabetic retinopathy [61], higher amounts of VEGF-A still need to be tested in this and/or other models, to precisely define the concentration range needed to activate aberrant cell proliferation or angiogenesis and the underlying molecular pathways. This evidence concerns the gene VEGFA and proliferative diabetic retinopathy.