In order to further determine how TRAP1 modulates the activation of Wnt/β-Catenin signaling, we monitored the enhancing effect of Wnt3A ligand on the activity of Wnt/β-Catenin pathway in HEK293T cells (Figure 1A,B)—a Wnt OFF cell line carrying a functional destruction complex [19]—and in CRC HCT116 cells, which are characterized by a β-Catenin mutation [20] (Figure 1C,D). The gene discussed is WNT3A; the disease is colorectal carcinoma.