In order to determine whether increased MHC-I expression affects tumor cell autonomous antigen presentation, we treated transferrin receptor-ovalbumin expressing E0771 (E0771-Ova) cells with MEKi prior to flow analysis using antibodies specific for total MHC-I (H2Kb) and MHC-I presenting the class-I-restricted ovalbumin antigen SIINFEKL (H2Kb-SIINFEKL)[20,21]. The gene discussed is TFRC; the disease is neoplasm.