Moreover, during IVM, treatment with 50 μg/ml NSE resulted in higher gene expression of Dnmt1 (1.4 ± 0.17 vs 0.56 ± 0.09, p = 0.0071), (1.24 ± 0.03 vs 0.54 ±0.03, p = 0.014) and Hdac1 (1.1 ± 0.12 vs 0.52 ±0.09, p = 0.0097), (1.06 ± 0.12 vs 0.52 ±0.04, p = 0.0065) levels in comparison to untreated oocytes in both control and PCOS mice, respectively (Figure 5). This evidence concerns the gene HDAC1 and polycystic ovary syndrome.