Percentage rates of fertilization, 2-cell, 8-cell, and blastocyst formation of control and PCOS mice oocytes cultured in maturation medium, supplemented with 0, 1, 50, and 100 μg/ml NSE. NES: Nigella sativa extract; PCOS: Polycystic ovary syndrome. Data are presented as Mean ± SEM. P-value are presented between two groups (Control versus PCOS) by student's t test. The gene discussed is ENO2; the disease is polycystic ovary syndrome.