MII %: The percentage of mature oocytes; Abnormal morphology %: The percentage of oocyte with abnormal morphology after maturation; NES: Nigella sativa extract; PCOS: Polycystic ovary syndrome. N: number of oocytes. Values are expressed as Mean ± SEM. P: P-value between each concentration group of NSE and control (0 μg/ml of NSE) by one-way ANOVA. This evidence concerns the gene ENO2 and polycystic ovary syndrome.