g.)for 4 weeks, we found that trilobatin significantly improved the deleterious effect induced by obesity on the phosphorylation of IRS1 at Ser 612 (Fig. 8a) and Ser 307 (Fig. 8b), and Akt at Thr 308 (Fig. 8c), but treatment with 10 mg/kg trilobatin for 4 weeks had no effect on the phosphorylation of AKT at Ser 473 (Fig. 8d) in the skeletal muscle tissue of ob/ob mice. The gene discussed is IRS1; the disease is obesity due to melanocortin 4 receptor deficiency.